Microwells to detect odors.

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United States scientists have developed a microfluidic-based platform that filters out hundreds of cells at a time and can be used to identify the behavior of cells that are rare in helping to stem cell research and cancer biology, they claim.

Ten of the hundreds of odorant molecules can be distinguished by the nose. Olfactory sensory neurons (OSNs) in the nasal odorant chemical signals translate into a unique neuronal signals which guide the behavior of many important. Odours activate odorant receptors (ORS) in which there are over 1000 types in mice. Each OSN showed only or in the type of OR, but due to large amounts is not possible to analyze the response during all types of odour-OR OSN using current techniques.

Albert Folch now at the University of Washington, Seattle and his team have developed a method to filter out nearly 20 000 single cells at a time on the unity of microwell. By using calcium imaging they were able to detect and analyze a number of odorant responses of rat OSNs simultaneously, clearly Folch, by enabling the cells with a rare pattern of responses found.

Folch compare several responses OSN with four odors of different fruits (vanilla, roses, berries and bananas) in one experiment. By using calcium imaging, the response of neurons identified by measuring changes in fluorescence signal. Folch explains that by using microwell to be placed on hundreds of OSNs in the camera visibility means that all the ORS should be represented in the sample. 'I believe that these microarrays are very powerful at finding the rare cells showing a particular pattern of response of which is not likely to be found with any reason' he said.

Tim Holy, who examined the olfactory system in rats at Washington University in St. Louis, United States, saying that 'This is an elegant new approach to record many neurons simultaneously maintained under stimulus conditions very well. "

Folch said that his team would eventually develop a system whereby cells are exposed to some odour in the gas phase, 'I expect, but can not prove, that the various problems with OSN survival and artifacts pembauan / adaptation comes from the fact bahwasannya odorant ( most of them dissolved in the water) just as if 'he added.

news
United States scientists have developed a microfluidic-based platform that filters out hundreds of cells at a time and can be used to identify the behavior of cells that are rare in helping to stem cell research and cancer biology, they claim.

Ten of the hundreds of odorant molecules can be distinguished by the nose. Olfactory sensory neurons (OSNs) in the nasal odorant chemical signals translate into a unique neuronal signals which guide the behavior of many important. Odours activate odorant receptors (ORS) in which there are over 1000 types in mice. Each OSN showed only or in the type of OR, but due to large amounts is not possible to analyze the response during all types of odour-OR OSN using current techniques.

Albert Folch now at the University of Washington, Seattle and his team have developed a method to filter out nearly 20 000 single cells at a time on the unity of microwell. By using calcium imaging they were able to detect and analyze a number of odorant responses of rat OSNs simultaneously, clearly Folch, by enabling the cells with a rare pattern of responses found.

Folch compare several responses OSN with four odors of different fruits (vanilla, roses, berries and bananas) in one experiment. By using calcium imaging, the response of neurons identified by measuring changes in fluorescence signal. Folch explains that by using microwell to be placed on hundreds of OSNs in the camera visibility means that all the ORS should be represented in the sample. 'I believe that these microarrays are very powerful at finding the rare cells showing a particular pattern of response of which is not likely to be found with any reason' he said.

Tim Holy, who examined the olfactory system in rats at Washington University in St. Louis, United States, saying that 'This is an elegant new approach to record many neurons simultaneously maintained under stimulus conditions very well. "

Folch said that his team would eventually develop a system whereby cells are exposed to some odour in the gas phase, 'I expect, but can not prove, that the various problems with OSN survival and artifacts pembauan / adaptation comes from the fact bahwasannya odorant ( most of them dissolved in the water) just as if 'he added.